Home > Free Sequencing > Free Sequencing

Free Sequencing

Cold Code (Murphyet al.2011) will sequence theFIRST TEN SPECIMENS of ONE SPECIES atNO COST to a contributor. Sequencing is restricted to mitochondrial COI (coxI). 

Each tissue sample must be accompanied by: 

·         species identification

·         collecting information locality (with GPS data)

·         voucher specimen number

·         collector(s)

·         and steward of the data relevant voucher data for each sample

·         we much prefer a royalty-free image of each species


We strongly encourage contributors to use the BOLD datasheet. [BOLD Handbook]


KIZ will hold the data for 3 yr from date of returning the sequence data or until they are published, whichever occurs first; other contributing facilities may have different guidelines. All data will go into BOLD as well as GenBank. 

Residual DNA will be banked securely at KIZ in the event that resequencing with new primers, verification or additional markers are needed. Residual tissue and DNA will not be distributed to any other researchers, including those at the host institution, without written permission of the steward. 

Upon request, Cold Code may cover shipping costs to China and provide tissue tubes for those who cannot afford them on their own. 

Investigators with large collections of tissues and limited funding may be brought to China either for training or for data gathering on their own at the expense of Cold Code. Researchers with adequate funding may come to China and avail themselves of the facility, which has two 96-well ABI 3730XL automated sequencers plus several formats of next-generation sequencers. 

For names, Cold Code will rely on the online databases Amphibian Species of the World and the Reptile Database. 

Whenever possible, specimens from the type locality should be submitted because of the great extent of cryptic genetic diversity and undescribed species (e.g. Vieiteset al.2009). 

Because of cryptic species, initially Cold Code will consider samples with >10% pairwise genetic distance to constitute a new species (candidate species; Vieites et al. 2009) for the purpose of providing the service. 

Legal collecting and shipping is required. For fieldwork, the steward of the tissue should possess and retain the associated paperwork. Cold Code will NOT bank the permits. 

Presently, the shipment of CITES-controlled material to China is complicated. This sequencing may be done outside China or PCR product can be sent to KIZ for sequencing as it is exempt from CITES regulations; contact Dr. Wen-zhi Wang ( for details. The speices for already barcoded could know from clicking " tool" to download "cheklist". If you have samples out of "cheklist" and not exceed10 samples, theSouthern China DNA Barcoding Center (SCDBC) can sequencing  for free. For extra specimens, only need pay CNY (Chinese Yuan)  60.00yuan for each.

Check here download checklist for free DNA barcoding.




Murphy, R.W., A.J. Crawford, A.M. Bauer, et al. 2013. Cold Code: the global initiative to DNA barcode amphibians and nonavian reptiles. Molecular Ecology Resources 13(2): 161-167.

Vieites DR, Wollenberg KC, Andreone F, et al. (2009) Vast underestimation of Madagascar‘s biodiversity evidenced by an integrative amphibian inventory. Proceedings of the National Academy of Sciences, 106, 8267-8272.