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1.Free  sequencing

Cold Code is being coordinated out of the Kunming Institute of Zoology (KIZ), Chinese Academy of Sciences. To facilitate Cold Code, the Kunming Institute of Zoology, Chinese Academy of Sciences will sequence COI for the first 10 specimens of a speciesat no cost to the steward of the tissues.

Presently, the shipment of CITES-controlled material to China is complicated. This sequencing may be done outside China or PCR product can be sent to KIZ for sequencing as it is exempt from CITES regulations; contact Dr. Wen-zhi Wang (wangwz@mail.kiz.ac.cn) for details.The speices for already barcoded could know from clicking " tool" to download "cheklist". If you have samples out of "cheklist" and not exceed 10 samples, theSouthern China DNA Barcoding Center (SCDBC) can sequencing  for free. For extra specimens,  only need pay CNY (Chinese Yuan)  60.00yuan for each.


 Check here download checklist for free DNA barcoding.


2. Primer 


Name

Ratio 

           sequence 5'-3'

Taxonomic Groups 

Reference

Regular primer pairs

RepCOI-F

 

TNTTMTCAACNAACCACAAAGA

Reptile

 Nagy et al. 2011

RepCOI-R

 

ACTTCTGGRTGKCCAAARAATCA

Reptile

 

 

 

 

 

 

chmf4

 

TYTCWACWAAYCAYAAAGAYATCGG

Amphibian

Che et al. 2011

chmr4

 

ACYTCRGGRTGRCCRAARAAT CA

Amphibian

 

 

 

 

 

 

CO1_CO1 

  1

TYTCWACWAAYCAYAAAGAYATTGG

Amphibian

Che et al. 2011

CO1_CO2 

  1

AYTCAACAAATCATAAAGATATTGG

Amphibian

 

CO1_CO3

  1

ACYTCYGGRTGACCAAARAAYCA

Amphibian

 

CO1_CO4

  1

ACYTCRGGRTGACCAAAAAATCA

Amphibian